Yq. Wang et al., REE BOUND POLYSACCHARIDES IN LEAVES OF DICRANOPTERIS-DICHOTOMA BY MAA, Science in China. Series B, Chemistry, life sciences & earth sciences, 41(3), 1998, pp. 266-271
The binding of REEs with polysaccharides (PSs) in the leaves of fern D
icranopteris dichotoma (DD) has been studied by molecular activation a
nalysis (MAA). The PSs of different solubilities in the leaves of DD,
which were named cold-water-soluble, hot-water-soluble, acidic-soluble
and basic-soluble PSs, were obtained using biochemical separation tec
hniques. After deproteinization and gel permeation chromatography sepa
ration, the absorption curves of the PSs were obtained by colorimetry.
The eight REEs (La, Ce, Nd, Sm, Eu, Tb, Yb,and Lu) were also detected
in the PSs by neutron activation analysis (NAA). The results obtained
showed that the REEs were bound firmly with the PSs in the leaves of
DD. The molecular weight measurement on Sephadex G-200 demonstrated th
at those PSs bound with REEs were mostly small molecular weight polysa
ccharides (10000 to 20000 u).