2 UNUSUAL AMINO-ACID SUBSTITUTIONS IN CYTOCHROME-B OF THE COLORLESS ALGA POLYTOMELLA SPP - CORRELATION WITH THE ATYPICAL SPECTRAL PROPERTIES OF THE B(H) HEME

The dithionite-reduced spectra of the purified be, complexes from the colorless alga Polytomella spp. and the closely related green alga Chl amydomonas reinhardtii were compared. The spectrum of the be, complex from C. reinhardtii showed a profile similar to those of the be, compl exes from other species;;In contrast, the be, complex from Polytomella spp. exhibits a double-peak spectrum in the alpha-band region, where the absorption bands of cytochrome c(1) and cytochrome b are completel y resolved. To further understand the molecular basis of these spectro scopic differences, the mitochondrial gene encoding cytochrome b of Po lytomella spp. was cloned, sequenced, and compared with that of C, I r einhardtii. The Polytomella spp. cytochrome Ib gene is 1113 bp long an d does nest contain introns. The deduced protein sequence exhibits 56% identity and 68% similarity with the cytochrome b of C. reinhardtii, and in a poylogenetic analysis it clearly affiliated with the b-type c ytochromes of C. reinhardtii and C. smithii. A comparison of the prima rily sequences of the Polytomella spp. cytochrome b with Other b-type cytochromes, and its analysis based on the structure featuring eight t ransmembrane stretches, allowed the identification of a tyrosine in po sition 114, which substitutes for a tryptophan present in all mitochon drial b-type cytochromes sequenced tea date. In addition, the primary sequence of the cytochrome b from Polytomella; spp. has a serine at po sition 36, instead of a nonpolar residue (alanine or leucine) found in all other species. In the proposed model for cytochrome b, both resid ues Tyr,,, and Ser,, are in close proximity to the high-potential b, h eme. The above data suggest that the polar residues Y-114 and S-36, ea ch one by itself or ill combination, may interact with heme b, of Poly tomella spp. and, thus, may be responsible for the unique spectroscopi c characteristics of cytochrome b. (C) 1998 Academic Press.