EXTRACTIONLESS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THEDETERMINATION OF DICLOFENAC IN HUMAN PLASMA AND URINE

An assay using reversed-phase high-performance liquid chromatography w ith ultraviolet detection, at 278 nm, was developed to measure diclofe nac in human plasma and urine at concentrations suitable for biopharma ceutical studies. Indomethacin was used as internal standard and separ ation was performed at 40-degrees-C on a C18 Spherisorb column with ac etonitrile-0.1 M sodium acetate (35:65, v/v) (pH 6.3) as mobile phase. The sample preparation is simple and rapid (extractionless), and the total run time is less than 5 min. The retention time is 2.8 min for d iclofenac and 3.6 min for indomethacin. The detection limit is 0.2 ug/ ml using a 20-mul loop.