Upper thermal tolerances were determined for four species of termite p
seudergates and nymphs using both a preset temperature method and a bi
oassay in which the temperature was increased at a known rate. Termite
s were acclimated at 28 degrees C for a minimum of 24h prior to being
placed into an oven preset to temperatures of 40 degrees C to 49 degre
es C, or placed into an oven at room temperature (ca. 25 degrees C) wh
ich was then raised at 1.0 degrees C/min. In preset bioassays, the low
est temperature that caused 100% mortality in all replicates was 42 de
grees C for Coptotermes formosanus Shiraki and 45 degrees C for Incisi
termes immigrans (Light). In temperature rate bioassays, upper lethal
limits (ULL) were 47.9 degrees C, 51.0 degrees C, 51.3 degrees C and 5
1.3 degrees C for C. formosanus, I. immigrans, Neotermes connexus Snyd
er, and Cryptotermes brevis (Walker), respectively. These elevated ULL
values could be due to a time-lag between the body temperature and th
e chamber temperature, or result from increased thermotolerance; both
of which are possibilities with gradually increasing temperatures. Act
ual lethal thresholds are likely to be lower than observed lethal tole
rances generated by the ULL method.