Several parameters were investigated to determine the effects of vitam
in C (AsA) on improvement of juvenile yellowtail quality for restockin
g. The dry pellets with different levels of L-ascorbyl-2-phosphate-Mg
(APM) (0, 400, 1000, and 2000 mg/kg diet) were formulated and fed to 5
00 juveniles (mean total length (TL): 3.6 cm) per treatment with dupli
cate tanks (500 1 round type). Test diets were fed three times a day a
t 25 degrees C water temperature for 20 days. After the 20-day trial,
10 to 20 juveniles from each treatment group were randomly sampled for
a test of 'air dive' and schooling behavior. The 'air dive' was condu
cted to determine the number of survivors after exposure to air while
the schooling behavior such as schooling pattern, swimming activity an
d individual distance were recorded using the video system and analyze
d by the computer graphic system. Survival was over 90% and there was
no sign of AsA deficiency in any group during the 20-day trial. There
was no effect of APM intake on growth. Liver AsA increased with increa
sing APM intake whereas that of brain AsA levelled off at 1000 mg APM,
The tolerance against air exposure seemed poor in fish fed on APM-fre
e diet although statistical significance was not detected (P > 0.05).
The schooling rate was significantly higher in fish fed on diets conta
ining 1000 or 2000 mg APM, It is speculated that juvenile yellowtail f
ed on diet containing more than 1000 mg APM would be able to show bett
er growth and survival under natural conditions after release. (C) 199
8 Elsevier Science B.V.