KANAMYCIN REVISITED - A COMBINED POTENTIOMETRIC AND SPECTROSCOPIC STUDY OF COPPER(II) BINDING TO KANAMYCIN-B

Coordination of Cu(II) to kanamycin B, an aminoglycosidic antibiotic, was studied by potentiometry and UV-Vis, circular dichroism and electr on paramagnetic resonance spectroscopies. Mononuclear complexes with s toichiometries ranging from CuH(3)A to CuH-2 A were found. Kanamycin B binds Cu(II) through nitrogen and oxygen donors of two terminal amino sugar rings, but the central deoxystreptamine moiety does not particip ate in the binding. The complex mediates oxidation of 2'-deoxyguanosin e by H2O2, very effectively. These properties may contribute to biolog ical properties of kanamycin B. (C) 1998 Elsevier Science S.A. All rig hts reserved.