ANTIBODY AND CYTOKINE RESPONSES IN EFFERENT LYMPH FOLLOWING VACCINATION WITH DIFFERENT ADJUVANTS

The cannulated efferent lymph node in sheep was used to examine the ef fect of different adjuvants on the antibody and cytokine responses fol lowing sub-cutaneous vaccination with a recombinant Taenia ovis antige n (45W). Vaccination with Quil A elicited relatively higher levels of IgM than did IFA or Al (OH)(3). In general, 45W specific IgG(1) and Ig G(2) titres were higher and maintained for longer periods of time in l ymph from sheep vaccinated with IFA and lower and shorter lived in ani mals which received the Al(OH)(3) based vaccine. Interferon-gamma was present within one day in efferent lymph from all sheep which received the Quil A formulation and in only one of the three sheep that receiv ed the II;A formulation GM-CSI; was only detected in lymph from sheep vaccinated with the IFA formulation. IL-8 was present in lymph prior t o vaccination and only animals which received the Quil A formulation h ad increased levels of IL-8 after vaccination. Neither of the inflamma tory cytokines IL-1 beta and TNF alpha were detected in efferent lymph from any animals in this study. This paper highlights the potential o f the lymphatic cannulation model for investigations of the in vivo ac tion of adjuvants, (C) 1998 Elsevier Science B.V. All rights reserved.