SIMPLE SEPARATION OF IMMUNOGLOBULIN FROM EGG-YOLK BY ULTRAFILTRATION

A water-soluble fraction (WSF) was prepared by filtering 10 times dilu ted yolk (pH 5.0) stored at 4-6 degrees C through Whatman No. 52 or No . 1 filter paper for delipidation. However, solid-phase delipidation u sing an octadecyl column after filtration through a cellulose powder c olumn was more practical for industrial application. After ultrafiltra tion (UF) of the WSF (pH 9.0, 1.5M NaCl), results showed: Amicon 80-85 % recovery, 74-99% purity; Harp 72-74% recovery, 81-84% purity; A/G 75 % recovery, 89% purity; Koch 88% purity.