ANALYSIS OF HYBRIDOMA CELL-CULTURE PROCESSES BY SDS GEL CAPILLARY-ELECTROPHORESIS AND MATRIX-ASSISTED LASER-DESORPTION IONIZATION-TIME-OF-FLIGHT MS/

SDS/gel capillary electrophoresis (SDS/gel CE) and matrix-assisted las er desorption ionization-time-of-flight MS (MALDI-TOF-MS) were employe d to analyse changes in the culture broth during batch and continuous cultivation of hybridoma cells, The stability of IgG was analysed by S DS/gel CE and capillary zone electrophoresis (CZE), The results obtain ed by the new analytical procedures reflect the changes in the cultiva tion conditions very well, indicating that these tools can be used to follow animal cell culture processes. A new technique to collect fract ions of very small volumes during the CZE separation is described, and the successful off-line coupling of CZE and MALDI-TOF-MS for the anal ysis of biotechnological processes is demonstrated.