A whole genome scan was undertaken in a granddaughter design comprisin
g 1158 progeny-tested bulls in order to map QTL influencing milk yield
and composition. In this paper we report the identification of a locu
s on the centromeric end of bovine Chromosome (Chr) 14, with major eff
ect on fat and protein percentage as well as milk yield. The genuine n
ature of this QTL was verified using the grand(2)-daughter design, tha
t is, by tracing the segregating QTL alleles from heterozygous grandsi
res to their maternal grandsons and confirming the predicted QTL allel
e substitution effect.