A SCREEN FOR INHIBITORS OF DNA RECOMBINATION - IDENTIFICATION OF 2 NEW SPIROSTANOL GLYCOSIDES FROM CHAMAEDOREA-LINEARIS

Two new glycosides have been isolated from the MeOH extract of the ste m wood and stem bark of an Ecuadorian plant Chamaedorea linearis, and their structures have been determined by spectroscopic means and X-ray analysis of the aglycone to be ulfate)]-25R,5alpha-spirostane-1beta,3 beta-diol[1] and -sulfate)]-25R,5alpha-spirostane-1alpha,3beta-diol [2 ). These compounds were identified in a screen for inhibitors of recom binational DNA repair. Cytotoxic activity was also demonstrated.