IN-SITU LOCALIZATION OF YERSINIA-ENTEROCOLITICA BY CATALYZED REPORTERDEPOSITION SIGNAL AMPLIFICATION

Aim-The sensitive detection of pathogenic Yersinia enterocolitica in p araffin embedded tissue sections by in situ hybridisation (ISH). Metho ds-Y enterocolitica infected cell lines, rat spleens, and patient biop sy specimens were used to compare conventional ISH, immune fluorescenc e assay (IFA) detection, and catalysed reporter deposition (CARD) sign al amplification ISH. Results-CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity of IFA. In cont rast to IFA, CARD-ISH preserved good tissue morphology. Conclusions-CA RD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular l ocalisation of the pathogen within the infected tissue.