''Water window'' soft X-ray has some advantages in the microimaging of
biological specimens. Soft X-ray holography can give us three-dimensi
onal images of biological cells in their natural condition. Quasi-mono
chromatic soft X-ray from the polymonochromatic synchrotron radiation
is obtained by a zone plate-pinhole linear monochromator at National S
ynchrotron Radiation Laboratory (NSRL) at Hefei. The pinhole is used t
o increase the temporal coherence of the soft X-ray, and also to incre
ase the spatial coherence of it. An in-line holographic experiment has
been finished with soft X-ray of main wavelength 4.5 nm and pinhole o
f diameter 0.03 mm. The magnified hologram is reconstructed with He-Ne
laser. A smaller pinhole is wanted to be used to improve the coherenc
e of the soft X-ray so as to improve the quality of the hologram.