NEW TECHNOLOGY TO EXAMINE AND IMPROVE THE PURITY OF HYBRID RICE WITH HERBICIDE-RESISTANT GENE

The herbicide resistant bar gene has been widely used as selectable ma rker genes in the study on plant genetic transformation. Owing to the integration of the gene into rice genome, transgenic rice was resistan t to the herbicide Basta. Therefore, selection of transgenic plant and genetic analysis became easier. In the studies, bar gene was introduc ed as a genetic marker gene into rice restore line variety of two-line or three-line. Combined with conventional breeding method, the good h erbicide resistant individual plant was derived as the new restore lin e for hybrid combination. After sprayed with Basta at seedling phase, the real hybrid rice that had herbicide resistance could grow normally , while the fake hybrid rice and weeds, having no bar gene, were kille d by herbicide. The method described above helped rapidly to examine a nd improve the purity of hybrid rice.