RAPID AND SENSITIVE DETECTION OF STAPHYLOCOCCUS SPECIES IN MILK BY ELISA BASED ON MONODISPERSE MAGNETIC PARTICLES

Staphylococcus spp. are the single most important cause of mastitis in dairy cows. Aiming for a rapid and sensitive method for detection of staphylococci, a magnetic bead-based enzyme-linked immunosorbent assay (ELISA) employing MAb for the detection of staphylococci in milk was developed. Pretreatment of the sample with lysostaphin increased the s ensitivity of the assay. The method is specific for most of staphyloco cci which cause mastitis in bovines, detects between 10(4)-10(5) organ isms per ml and takes three hours. Thus, the principle for staphylococ ci ELISA based on monodisperse magnetic beads is a way for rapid detec tion of staphylococci in milk and possibly also for detecting staphylo cocci by direct testing of other clinical specimens. The system may be automated. (C) 1998 Elsevier Science B.V. All rights reserved.