EXPERIMENTAL RABIES IN BUFFALO CALVES - A PATHOGENETIC APPROACH ON EARLY SPREAD OF STREET RABIES VIRUS

Buffalo calves were experimentally inoculated with 1 25 462 MICLD50 of street rabies virus via linear laceration on the muzzle. Analysis of tentative progression in quantum and direction of rabies virus indicat ed that hippocampus. cerebellum, pons and medulla oblongata are much m ore dependable sites to attempt isolation of rabies virus in buffalo s pecies especially when a rabid buffalo has been killed during early ph ase of the disease. On histopathological examination, typical non-supp urative encephalo-myelitis was observed. The central neural transport mechanism of rabies virus has been monitored by specific immunofluores cence, histopathological findings. Seller's staining and mice inoculat ion tests on various central nervous tissues. Ocular involvement in th e form of conjunctivitis was observed among the early symptoms.