STABLE TRANSFORMATION OF HI-II MAIZE USING THE PARTICLE INFLOW GUN

Embryogenic type II callus, initiated from cultured immature zygotic e mbryos of the maize line Hi-II, was transformed by microprojectile bom bardment using a particle inflow gun. This highly regenerable maize li ne was transformed with the plasmid pAHC25, a dual expression vector c ontaining the uidA reporter gene and the bar selectable marker gene, b oth under the control of the maize ubiquitin promoter: Transgenic plan ts were obtained from 10 independent transformation events. Transmissi on of the bar and uidA genes to the progeny of three of these lines wa s demonstrated by Southern blot analysis and by transgene expression.