QUANTIFICATION BY RADIOIMMUNOASSAY OF ADIPOKINETIC HORMONE-I IN NEURAL TISSUES IN THE HEAD OF LOCUSTA-MIGRATORIA

1. Using a [H-3]AKH-I probe, a radioimmunoassay has been developed whi ch is specific for adipokinetic hormone-I (AKH-I). 2. Age-related chan ges occur in the AKH-I content of male and female corpora cardiaca: le vels are ca 200 pmol per pair of glands at emergence, increasing to ca 800 pmol by day 30, but the patterns of increase are complex and diff er temporally between the sexes. 3. No age-related changes are found i n the amounts of AKH-I in brains from males or females, with total amo unts < 20 pmol/brain. 4. No relationship was found between oocyte leng th and the AKH-I content of female corpora cardiaca. 5. Reverse phase HPLC confirms that the immunoreactive material in extracts of male and female brains has the same retention time as AKH-I. 6. AKH-I was not detected in suboesophageal ga