RHODIOLA ROSEA IN-VITRO CULTURE - PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTION

Various Rhodiola rosea organs and tissues from in vitro culture were s tudied in two areas: searching for the biological active four phenolic compounds and measurement of antioxidant activity of dry residues of EtOH-extracts soluble in PBS using chemiluminescence method. For phyto chemical investigation HPLC method was used. Salidroside was typical f or organs of intact plant, rosavin for roots of different origin and s hoots of intact plants, triandrin was more typical for tissue cultured in vitro, but it was found in all studied samples. Caffeic acid was d etected mainly in green and yellow lines of callus, less in other Rhod iola rosea plant material. The highest antioxidant activity showed the extract from 1,5-years-old callus of green and yellow line, which con tained caffeic acid and triandrin. Further observations are in progres s.