P. Lindberg et al., FIBER-OPTIC-BASED UV-VISIBLE ABSORBENCY DETECTOR FOR CAPILLARY-ELECTROPHORESIS, UTILIZING FOCUSING OPTICAL-ELEMENTS, Journal of chromatography, 809(1-2), 1998, pp. 181-189
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A compact and flexible fiber-optic cell for absorbance detection in ca
pillary electrophoresis (CE) has been developed. Optical fibers with a
large core diameter were combined with focusing optics and a circular
aperture, the size of the aperture being matched to the I.D. and O.D.
of the separation column, in order to maximize light throughput and r
educe stray light. The detection limit for mesityl oxide was 1.5 mu M
for a 100-mu m I.D. column, and the linear dynamic range was larger th
an 1000 for columns from 100-mu m I.D. down to 30-mu m I.D. The design
is suitable for high-speed CE, utilizing short columns. (C) 1998 Else
vier Science B.V. All rights reserved.