R. Metz et al., SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ARBIDOL, A NEW ANTIVIRAL COMPOUND, IN HUMAN PLASMA, Journal of chromatography, 810(1-2), 1998, pp. 63-69
Citations number
3
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A highly sensitive and selective HPLC method was developed and validat
ed for the determination of arbidol in human plasma. The method involv
es the liquid-liquid extraction of drug and internal standard from pla
sma with tert.-butyl methyl ether followed by evaporation and reconsti
tution in mobile phase. UV detection was done at 315 nm. The limit of
quantification for arbidol in plasma was 0.005 mu g/ml. Linearity in p
lasma was proven over the whole calibration range (10.2-0.005 mu g/ml)
. The method was validated according to GLP guidelines and its suitabi
lity was demonstrated by analysis of samples from a pharmacokinetic st
udy. (C) 1998 Elsevier Science B.V. All rights reserved.