ELECTROCHEMICAL DETECTION OF MICROCYSTINS, CYANOBACTERIAL PEPTIDE HEPATOTOXINS, FOLLOWING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
MERILUOTO J KINCAID B SMYTH MR WASBERG M
Citation
J. Meriluoto et al., ELECTROCHEMICAL DETECTION OF MICROCYSTINS, CYANOBACTERIAL PEPTIDE HEPATOTOXINS, FOLLOWING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 810(1-2), 1998, pp. 226-230
Citations number
31
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
810
Issue
1-2
Year of publication
1998
Pages
226 - 230
Database
ISI
SICI code
Abstract
A novel amperometric HPLC detection method for the cyanobacierial (blu e-green algal) peptide toxins microcystin-LR, -YR and -RR was develope d. Purified microcystins and cyanobacterial extracts were chromatograp hed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reacti ons at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins. (C) 1 998 Elsevier Science B.V. All rights reserved.