COMPLETE NUCLEOTIDE-SEQUENCE OF THE HSD PLASMID PECO29 AND IDENTIFICATION OF ITS FUNCTIONAL REGIONS

The complete nucleotide sequence of the Hsd plasmid pECO29 has been de termined. The plasmid DNA consists of 3895 base pairs. These include 4 genes and 5 sites. Two genes encoding the proteins (restriction endon uclease and DNA methyltransferase) have been fully characterized. The pECO29 comprises a ColEl-type replication system coding for untranslat ed genes RNAI and RNAII, the emr recombination site containing palindr omic sequences and involved in stable maintenance of the plasmid, two pseudo oriT sites homologous to the oriT site of R64 and F plasmids, a s well as the born locus of a ColEl-like plasmid. There are no genes i nvolved in the mobilization of pECO29 plasmid. (C) 1998 Elsevier Scien ce B.V. All rights reserved.