COMPLEMENTATION CLONING OF NEMO, A COMPONENT OF THE I-KAPPA-B KINASE COMPLEX ESSENTIAL FOR NF-KAPPA-B ACTIVATION

Citation
S. Yamaoka et al., COMPLEMENTATION CLONING OF NEMO, A COMPONENT OF THE I-KAPPA-B KINASE COMPLEX ESSENTIAL FOR NF-KAPPA-B ACTIVATION, Cell, 93(7), 1998, pp. 1231-1240
Citations number
28
Categorie Soggetti
Biology,"Cell Biology
Journal title
CellACNP
ISSN journal
00928674
Volume
93
Issue
7
Year of publication
1998
Pages
1231 - 1240
Database
ISI
SICI code
0092-8674(1998)93:7<1231:CCONAC>2.0.ZU;2-Y
Abstract
We have characterized a flat cellular variant of HTLV-1 Tax-transforme d rat fibroblasts, 5R, which is unresponsive to all tested NF-kappa B activating stimuli, and we report here its genetic complementation. Th e recovered full-length cDNA encodes a 48 kDa protein, NEMO (NF-kappa B Essential MOdulator), which contains a putative leucine zipper motif . This protein is absent from 5R cells, is part of the high molecular weight I kappa B kinase complex, and is required for its formation. In vitro, NEMO can homodimerize and directly interacts with IKK-2. The N EMO cDNA was also able to complement another NF-kappa B-unresponsive c ell line, 1.3E2, in which the protein is also absent, allowing us to d emonstrate that this factor is required not only for Tax but also for LPS, PMA, and IL-1 stimulation of NF-kappa B activity.