DIGITAL IMAGING MICROSCOPY OF LIVING CELLS

Fluorescence microscopy has undergone a resurgence in interest followi ng the discovery of green-fluorescent protein (GFP) and its increasing rise in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be u sed to produce high-resolution three-dimensional images of samples lab elled with GFP, or other fluorochromes, using simple instrumentation a nd image-restoration software.