3 BIRTHS AFTER PREIMPLANTATION GENETIC DIAGNOSIS FOR CYSTIC-FIBROSIS WITH SEQUENTIAL FIRST AND 2ND POLAR BODY ANALYSIS

OBJECTIVE: The purpose of this study was to determine the accuracy and feasibility of sequential polar body removal and analysis for preimpl antation genetic diagnosis of mendelian disorders. STUDY DESIGN: Three couples with risk factors for cystic fibrosis had preimplantation gen etic diagnosis with the use of sequential polar body analysis. After s timulation, oocytes were harvested and the first polar bodies were rem oved and analyzed on the day of aspiration. The following day, after f ertilization, the second polar bodies were aspirated. Only embryos kno wn to have inherited the normal maternal allele were transferred. RESU LTS: All three couples had successful pregnancies resulting in the bir ths of unaffected infants. CONCLUSIONS: Preimplantation diagnosis with the use of sequential polar body removal is feasible and can prevent the establishment of genetically abnormal pregnancies for couples at r isk.