EXAMINATION OF THE PIPETTING VARIATION BETWEEN INDIVIDUALS PERFORMINGRANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS

Authors
MCEWAN NR BAKHT J CECCHINI E COULTART A GERI C MCDONALD FM MCDONALD MS RAHMAN NNA WILLIAMSON G
Citation
Nr. Mcewan et al., EXAMINATION OF THE PIPETTING VARIATION BETWEEN INDIVIDUALS PERFORMINGRANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS, Journal of microbiological methods, 32(3), 1998, pp. 213-215
Citations number
7
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
32
Issue
3
Year of publication
1998
Pages
213 - 215
Database
ISI
SICI code
0167-7012(1998)32:3<213:EOTPVB>2.0.ZU;2-5
Abstract
The similarity between random amplified polymorphic DNA (RAPD) profile s generated by nine experimenters is presented. Despite simultaneously using the same pipetts, the same solutions and the same thermocycler, no two RAPD profiles generated identical banding patterns. This resul t suggests that before using a particular primer, it is essential that all experimenters should obtain a reference profile for their own wor k, rather than comparing it to one generated by another researcher. (C ) 1998 Elsevier Science B.V.