The response of river water microbial communities to chemical compound
s was monitored under laboratory conditions using aniline as a model.
Bacteria were collected from unpolluted and polluted sites. Bacterial
abundance (plate and total direct counting) and its relation to anilin
e biodegradation was examined. Colony hybridization with 16S rRNA olig
onucleotide probes was used to study the changes in microbial communit
y structure during biodegradation of aniline. The changes in bacterial
abundance and community structure were related to biodegadation of an
iline. Burkholderia-Pseudomonas (rRNA group III), an authentic Alcalig
enes group became dominant despite the initial differences in the micr
obial communities, suggesting that these genera are the main aniline d
egraders in the aquatic environment.