TYPING OF SALMONELLA-ENTERITIDIS OF DIFFERENT PHAGE TYPES OF PCR FINGERPRINTING

Fifty-nine isolates of Salmonella spp. were typed by PCR fingerprintin g using three single primers: ERIC2, M13 and OPS-19. First, their disc rimination power in a group of nine different serotypes were studied a nd considerable differences in the band patterns were obtained. Furthe r, a panel of 51 isolates of Salmonella enteritidis with eight differe nt phage types were analysed with the three primers. The discriminatin g power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types o f Salm. enteritidis, but not all of them were differentiated.