NOTE - PURIFICATION AND CHARACTERIZATION OF THE BACTERIOCIN PSVP-10 PRODUCED BY PSEUDOMONAS SP

Authors
HUBERT E LOBOS O BREVIS P PADILLA C
Citation
E. Hubert et al., NOTE - PURIFICATION AND CHARACTERIZATION OF THE BACTERIOCIN PSVP-10 PRODUCED BY PSEUDOMONAS SP, Journal of applied microbiology, 84(5), 1998, pp. 910-913
Citations number
14
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Journal of applied microbiology → ACNP
ISSN journal
13645072
Volume
84
Issue
5
Year of publication
1998
Pages
910 - 913
Database
ISI
SICI code
1364-5072(1998)84:5<910:N-PACO>2.0.ZU;2-U
Abstract
A bacteriocin (bacteriocin PsVP-10) produced by Pseudomonas sp. R-10 w as purified by a simple method that included an extraction of the bact eriocin with chloroform, followed by cation exchange chromatography. T he purity of the bacteriocin was verified by RP-HPLC. It is a peptide of 2.4 kDa, very stable to heat, to proteolytic enzymes and to pH. It presents a very broad spectrum of antimicrobial activity against Gram- positive and Gramnegative bacteria.