IMMUNOGLOBULIN SUPERFAMILY MEMBERS GP65 AND GP55 - TISSUE DISTRIBUTION OF GLYCOFORMS

Gp65 and gp55 are immunoglobulin superfamily members produced by alter native splicing of the same gene transcript, and originally identified as components of synaptic membranes. A monoclonal antibody specific f or gp65 and gp55 has been used to detect immunoreactive species in a n ide range of tissues. All immunoreactive species bind to concanavalin A and deglycosylation studies show that in all tissues tested other th an brain the immunoreactive species are derived from gp55. HEK cells t ransfected with gp65 or gp55 express different glycoforms from brain s howing that the pattern of glycosylation of these molecules is depende nt upon the cell type in which they are expressed. (C) 1998 Federation of European Biochemical Societies.