DIFFERENTIAL BINDING OF HUMAN BLOOD-GROUP SD(A-) TAMM-HORSFALL GLYCOPROTEINS WITH DOLICHOS-BIFLORUS AND VICIA-VILLOSA B-4 AGGLUTININS() ANDSD(A)

The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm-Horsf all glycoproteins (THGPs) with respect to four GalNAc specific aggluti nins were studied by quantitative precipitin assay (QPA) and enzyme li nked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bo und well with Dolichos biflorus (DB,I) and Vicia villosa-B-4 (VVA-B-4) , while Sd(a-) THGP reacted poorly with these two lectins, Neither Sd( a+) nor Sd(a-) THGPs reacted with two other GalNAc alpha-anomer specif ic lectins: Codium fragile subspecies tometosoides and Artocarpus inte grifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B-4 and na tive Sd(a+)THGP-DBA through GalNAc beta --> was confirmed by inhibitio n assay, These results demonstrate that DBA and VVA-B-4 are useful rea gents to differentiate between Sd(a+) and Sd(a-) THGP. (C) 1998 Federa tion of European Biochemical Societies.