RICE CALLUS GROWTH, PROLINE CONTENT AND ACTIVITY OF PROLINE AND IAA OXIDASE UNDER THE INFLUENCE OF HYDROXYPROLINE AND NACL

Calli of salt tolerant (Bhoora rata) and salt susceptible (GF(11)) ric e varieties were cultured on Linsmaeir and Skoog's medium containing L D50 concentration of NaCl (200 mM) and hydroxyproline (10 mM). Growth, proline content and activity of proline and IAA oxidases of the cultu red tissues were determined at the end of 0, 2, 4, and 6 weeks of incu bation. Hydroxyproline resistant calli of both rice varieties when cul tured on Linsmaeir and Skoog's medium containing hydroxyproline and Na Cl showed increased dry weight and proline content as compared to NaCl stressed calli. The levels of proline and IAA oxidases were also low in the hydroxyproline resistant calli.