CROSS-SPECIES REACTIVITY OF A MONOCLONAL-ANTIBODY AGAINST GLUTATHIONE-S-TRANSFERASE FUSION PROTEIN OF HUMAN BETA-2-ADRENERGIC RECEPTOR

The purpose of the present study was to produce and characterize a mon oclonal antibody against human beta 2-adrenergic receptor. Male BALB/c mice were immunized with glutathione S-transferase (GST) fusion prote in of the C-terminal portion of the human beta 2-adrenergic receptor w hich was expressed in E.Coli. The immunized splenocytes were fused wit h myeloma SP2/0-Ag14 cells and the resulting monoclonal antibody was n amed as mAb beta C02. The monoclonal antibody beta C02 was determined as IgM subtype and then purified by anti-mouse IgM-agarose affinity ch romatography. The results of ELISA, Western blot, and immunocytochemis try showed that mAb beta C02 recognized human beta 2-adrenergic recept or in the beta 2-adrenergic receptor-GST fusion protein and human epid ermoid carcinoma cell line A431 with highly specific immunoreactivity. In addition, mAb beta C02 showed cross-species reactivity against bet a-adrenergic receptor of hamster lung and rat brain as revealed by We stern blot and immunohistochemistry. The monoclonal antibody beta C02 may provide useful tools for the study of the beta-adrenergic receptor of human and other species including rats.