CRYSTALS OF RIBOSOMAL-PROTEIN L1 FROM A HYPERTHERMOPHILIC ARCHAEON METHANOCOCCUS-JANNASCHII

Crystallographic studies of ribosomal proteins from bacteria progresse d rapidly during the last decade, though the structures of ribosomal p roteins from other kingdoms have not yet been published. Here we descr ibe crystalls of archaeal ribosomal protein L1 from Methanococcus jann aschii. The protein crystals were grown in 10% PEG 10 K, 50 mM Hepes-H Cl (pH 7.5) in hanging drops equilibrated against 33% PEG 10 K, 100 mM Hepes-HCl (pH 7.5). The crystals diffract to at least 2.5 Angstrom re solution and belong to the space group P1 with cell parameters a=34.09 Angstrom, b=39.39 Angstrom, c=55.84 Angstrom, alpha=83.65 degrees, be ta=80.38 degrees, gamma=75.37 degrees.