Mj. Hemmer et al., COMPARISON OF 3 HISTOLOGICAL FIXATIVES ON THE IMMUNOREACTIVITY OF MAMMALIAN P-GLYCOPROTEIN ANTIBODIES IN THE SHEEPSHEAD MINNOW, CYPRINODON-VARIEGATUS, The Journal of experimental zoology, 281(4), 1998, pp. 251-259
Mammalian P-glycoprotein is a highly conserved integral membrane prote
in functioning as an energy-dependent efflux pump which decreases the
concentration of certain lipophilic aromatic compounds entering the ce
ll by diffusion. Expression of such a transporter in teleost species c
ould play a significant role in conferring resistance to fish populati
ons exposed to xenobiotic stressors and may serve as a potential indic
ator of species at risk for certain environmental contaminants. In pre
vious studies we demonstrated that a strong correlation existed betwee
n corresponding mammalian and teleost tissues showing immunoreactivity
to specific mammalian P-glycoprotein antibodies. In the present study
, comparisons of staining pattern, intensity, and tissue specificity b
etween tissues treated in Bouin's, Dietrich's and Lillie's histologica
l fixatives were determined in the sheepshead minnow, Cyprinodon varie
gatus, using monoclonal antibodies C219, C494, JSB-1 and polyclonal an
tiserum MDR(Ab-1). Immunoreactivity of these antibodies was found to b
e fixative-dependent. Results are presented illustrating the different
ial staining patterns and tissue specificity observed for each tissue
type, fixative, and antibody combination. Our data indicate tissue fix
ation has a significant impact on P-glycoprotein antibody immunoreacti
vity in teleost tissues and must be considered in the comparison and i
nterpretation of results. J. Exp. Zool. 281:251-259, 1998. (C) 1998 Wi
ley-Liss, Inc.dagger.