Gas exchange responses to lightflecks of different frequency and durat
ion were studied in mature grapevine leaves. Light-response curves und
er intermittent light showed lower carbon exchange rate (CER) than con
tinuous light at non-saturating PFD levels (10-500 mu mol m-2s-1). Whi
te-dark and white-green alternating light of equal bright and shade pe
riods at 0.33-5.33 Hz systematically resulted in improved light utiliz
ation as compared to high continuous light. Transpiration (T) was slig
htly more limited than the CER under intermittent light, resulting in
higher water use efficiency. No differences in carboxylation efficienc
y were found by comparing the high continuous light treatment to white
-dark lightflecks at 0.33 and 2.67 Hz. Our results show that, under sh
ort-term alternating light, CER of the grapevine leaf fully responds t
o the mean PFD level resulting from light oscillations.