IN-VITRO METHOD TO SCREEN GRAPEVINE GENOTYPES FOR TOLERANCE TO LIME-INDUCED CHLOROSIS

Herbaceous nodes of four grapevine genotypes with different tolerance to lime-induced chlorosis (V. berlandieri x V. rupestris 140 Ru; V. be rlandieri x V. riparia SO 4; V, riparia MICH. Gloire de Montpellier; V . vinifera L. cv. Chardonnay) were cultured in vitro. The effects of t hree levels of FeNaEDTA (5, 15, 30 mg/l) and of four mixtures of iron and bicarbonate in the MS medium were compared and chlorosis rating, f errous iron content of the leaves and fresh weight of the plantlets we re assayed. The chlorosis rating of the tested genotypes ranked accord ing to their known degree of tolerance/susceptibility to lime-induced chlorosis.