BETA-GALACTOSIDASE IN IMMOBILIZED CELLS OF GHERKIN CUCUMIS-SATIVUS L

Authors
STANO J NEMEC P BEZAKOVA L KAKONIOVA D KOVACS P NEUBERT K LISKOVA D ANDRIAMAINTY F MICIETA K
Citation
J. Stano et al., BETA-GALACTOSIDASE IN IMMOBILIZED CELLS OF GHERKIN CUCUMIS-SATIVUS L, Acta Biochimica Polonica, 45(2), 1998, pp. 621-626
Citations number
26
Categorie Soggetti
Biology
Journal title
Acta Biochimica Polonica → ACNP
ISSN journal
0001527X
Volume
45
Issue
2
Year of publication
1998
Pages
621 - 626
Database
ISI
SICI code
0001-527X(1998)45:2<621:BIICOG>2.0.ZU;2-3
Abstract
Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. beta-Galactosidase showe d pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzym e catalysed hydrolysis was linear for 3 h with 60-68% conversion of th e substrate. The cells characterized by high beta-galactosidase activi ty and stability on long-term storage showed valuable technological pr operties.