MAPPING OF PORCINE GENES BELONGING TO 2 DIFFERENT CYTOCHROME-P450 SUBFAMILIES

Three cDNA clones from a porcine small intestine cDNA library, tentati vely identified as transcripts of two different cytochrome P450 (CYP) gene subfamilies, were sequenced and mapped. One of the clones was fou nd to comprise an open reading frame of 503 amino acids and showed up to an 80% similarity to genes from the CYP3A subfamily. Translation of the other two cDNA clones revealed high similarity to genes from the CYP2C subfamiliy. One of these clones was truncated, lacking a part of the 5' end of the coding region, whereas the other encodes a putative pseudogene. The CYP3A gene was mapped cytogenetically by means of a p ig/rodent somatic hybrid cell panel to pig chromosome 3, in the region p16-p17 or p11. The CYP2C pseudogene was mapped genetically to pig ch romosome 14, using the PiGMaP shared reference pedigrees. The localisa tions provide information for the human/pig comparative map.