A NEW TECHNIQUE FOR POSTEMBEDDING IMMUNOGOLD LOCALIZATION OF VASOPRESSIN IN THE RAT MEDIAN-EMINENCE

Authors
PENG QL ZHANG MH RICHTER HP BOCK R
Citation
Ql. Peng et al., A NEW TECHNIQUE FOR POSTEMBEDDING IMMUNOGOLD LOCALIZATION OF VASOPRESSIN IN THE RAT MEDIAN-EMINENCE, Acta histochemica, 95(1), 1993, pp. 75-78
Citations number
10
Categorie Soggetti
Cytology & Histology
Journal title
Acta histochemica → ACNP
ISSN journal
00651281
Volume
95
Issue
1
Year of publication
1993
Pages
75 - 78
Database
ISI
SICI code
0065-1281(1993)95:1<75:ANTFPI>2.0.ZU;2-S
Abstract
A modified cryosubstitution method is described which simultaneously p reserves the ultrastructure and antigenicity of the osmium-sensitive n europeptide vasopressin in rat median eminences. The organs were aldeh yde-fixed, osmicated, and embedded in Epon. Thin sections were no long er needed to be etched and the titer of primary antiserum was near to the light microscopic level. Furthermore ice crystallization could not be observed.