CHARACTERIZATION OF PROTEASE(S) IN ADULTS OF TRICHURIS-GLOBULOSA (NEMATODA, TRICHURIDAE)

The activity of protease(s) has been observed separately in male and f emale Trichuris globulosa. Different fractions showed optimum protease activity at 37 degrees C, pH 9.5 and 1.5 mg casein concentration. Exc retory/secretory (E/S) products showed maximum protease activity, whic h might be helpful in the degradation of mucosal tissue of the host gu t. Male parasites appear to feed extensively on connective tissue coll agen/elastin, while females feed on both connective tissue and body/ce llular fluids. Inhibition/activation studies revealed the presence of four kinds of protease in the E/S products, cytosolic and membrane-bou nd fractions performing different functions.