Ma. Dunn et al., A LOW-TEMPERATURE-RESPONSIVE TRANSLATION ELONGATION FACTOR-1-ALPHA FROM BARLEY (HORDEUM-VULGARE L), Plant molecular biology, 23(1), 1993, pp. 221-225
A cDNA clone (pBLT63) encoding a protein synthesis elongation factor 1
alpha (EF-1alpha) was isolated from a low-temperature winter barley sh
oot meristem library by differential screening. The nucleotide sequenc
e of the coding region of the low-temperature-induced barley gene show
s very high homology with two EF-1alpha plant genes from tomato and Ar
abidopsis. The barley genome contains an EF-alpha gene family situated
on the short arm of chromosome 2 and the long arm of chromosome 5.