VARIATIONS OF PROTEIN PROFILES UPON SHIFTS IN INORGANIC CARBON REGIMEIN THE CYANOBACTERIUM SYNECHOCYSTIS PCC6803

Reversible changes between limiting and high inorganic carbon regimes ir Synechocystis PCC6803 maintained overall protein synthesis at a hig h level. The protein patterns of cells during or after adaptation to t hese regimes were detected by pulse labelling and identification after one-dimensional and two-dimensional electrophoreses. Several particul ar patterns were observed. Proteins specific for either the high or th e limiting carbon conditions could be identified. The protein profiles of cells having reached the stationary phase of growth clearly differ ed from those of their exponentially growing counterparts. Accumulatio n of Rubisco, the key enzyme of the Calvin cycle, was not modified in the conditions tested. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.