PARTIAL-PURIFICATION AND CHARACTERIZATION OF RALF40I, A CLASS-II RESTRICTION-ENDONUCLEASE FROM RUMINOCOCCUS-ALBUS F-40, WHICH RECOGNIZES AND CLEAVES 5'- GATC-3'/
T. Miyagi et al., PARTIAL-PURIFICATION AND CHARACTERIZATION OF RALF40I, A CLASS-II RESTRICTION-ENDONUCLEASE FROM RUMINOCOCCUS-ALBUS F-40, WHICH RECOGNIZES AND CLEAVES 5'- GATC-3'/, FEMS microbiology letters, 164(1), 1998, pp. 215-218
Restriction endonuclease RalF40I was purified from cell-free extracts
of the rumen cellulolytic bacterium Ruminococcus albus F-40 by heparin
-Sepharose chromatography. The preparation was active only on DNA subs
trates that were nor Dammethylated. RalF40I recognizes the 4-bp palind
rome, 5'-/GATC-3', and cleaves DNA at the 5' side of G in the sequence
, producing 5' tetranucleotide protruding ends. RalF40I is a class II
restriction endonuclease and an isoschizomer of MobI and DpnII. (C) 19
98 Federation of European Microbiological Societies. Published by Else
vier Science B.V. All rights reserved.