PARTIAL-PURIFICATION AND CHARACTERIZATION OF RALF40I, A CLASS-II RESTRICTION-ENDONUCLEASE FROM RUMINOCOCCUS-ALBUS F-40, WHICH RECOGNIZES AND CLEAVES 5'- GATC-3'/

Citation
T. Miyagi et al., PARTIAL-PURIFICATION AND CHARACTERIZATION OF RALF40I, A CLASS-II RESTRICTION-ENDONUCLEASE FROM RUMINOCOCCUS-ALBUS F-40, WHICH RECOGNIZES AND CLEAVES 5'- GATC-3'/, FEMS microbiology letters, 164(1), 1998, pp. 215-218
Citations number
9
Categorie Soggetti
Microbiology
Journal title
ISSN journal
03781097
Volume
164
Issue
1
Year of publication
1998
Pages
215 - 218
Database
ISI
SICI code
0378-1097(1998)164:1<215:PACORA>2.0.ZU;2-N
Abstract
Restriction endonuclease RalF40I was purified from cell-free extracts of the rumen cellulolytic bacterium Ruminococcus albus F-40 by heparin -Sepharose chromatography. The preparation was active only on DNA subs trates that were nor Dammethylated. RalF40I recognizes the 4-bp palind rome, 5'-/GATC-3', and cleaves DNA at the 5' side of G in the sequence , producing 5' tetranucleotide protruding ends. RalF40I is a class II restriction endonuclease and an isoschizomer of MobI and DpnII. (C) 19 98 Federation of European Microbiological Societies. Published by Else vier Science B.V. All rights reserved.