USE OF A MICROELECTRODE SYSTEM AS A TOOL OF THE QUANTITATIVE APPLICATION OF WOUND TO THE CELL-TO-CELL JUNCTIONAL MEMBRANE OF CULTURED TOBACCO CELLS

Cultured tobacco cell line BY-2 is a promising material for the study about the cell-to-cell junctional membrane (CJM), because it forms a c hain of several cells. This study proposes the use of a microelectrode system in the quantitative application of mechanical wound to particu lar CJM of BY-2 cells. After the intercellular electroporation by puls ing electric field (pulse height, P-H=0, 10,50, or 340V; pulse width, P-W=90 msec; interval between pulses, P-I=80 msec), the viability of t he cells was checked. When P-H was 10V, one of ten cells maintained th e viability and divided within 12 hr. Based on a linear electric circu it model, the pulse height actually working on CJM (V-CJM) was estimat ed as 2.53V.