COMPARISON BETWEEN ONCE-A-DAY VS TWICE A DAY G-CSF FOR MOBILIZATION OF PERIPHERAL-BLOOD PROGENITOR CELLS (PBPC) IN NORMAL DONORS FOR ALLOGENEIC PBPC TRANSPLANTATION

Despite the wide use of G-CSF for mobilization of PBPC the best dose a nd schedule of G-CSF has not been definitively established. Zn this st udy we have compared three different schedules of G-CSF for mobilizati on of PBPC in normal donors including a single daily dose of 10 mu g/k g/day for 5 days (21 donors) and doses of 6 (21 donors) or 8 mu g/kg/1 2 h (6 donors) for 5 days, We demonstrate that G-CSF at doses of 6 and 8 mu g/kg/12 h mobilizes significantly more CD34(+) cells/ml of blood (83.3 +/- 6.7 and 121 +/- 6.9, respectively) than 10 mu g/kg/day (71. 6 +/- 6.5), Mobilization with 6 or 8 mu g/kg/12 h of G-CSF was also as sociated with collection of significantly more CD34(+) cells in compar ison,vith 10 mu g/kg/24 h (2.24 +/- 1.2 and 2. 46 +/- 1.22 vs 1.15 +/- 0.8 CD34(+) cells/kg of donor/blood volume), PBPC collection was asso ciated with a significant decrease in platelet count which was not sig nificantly different between the three groups, Ten days after the last PBPC collection platelet counts were within normal limits while there was a decrease in WBC and ANC, We conclude that G-CSF administered ev ery 12 h at doses of 6 mu g/kg provides better CD34(+) cell yield than 10 mu g/kg once a day in normal donors which may translate into a dec rease in the number of aphereses required to obtain enough numbers of CD34(+) cells for allogeneic PBPC transplant.