PRODUCTION OF SINGLE-CELL PROTEIN FROM SA CCHAROMYCES-LIPOLYTICA GROWING ON EMULSION OF CUTTING OIL

Culture media containing used emulsion of cutting oil (ECO) as carbon source were fermented by Saccharomyces lipolytica CCT 0913 for single cell protein (SCP) production and chemical oxygen demand (COD) reducti on. Two media were used, the first containing (NH4)(2)SO4 as nitrogen source with concentration in the range 2.5 to 15.0 g/l, and the second containing NH4Cl with concentration range 0.1 to 1.0 g/l. Fermentatio ns were carried out in Erlenmeyer flasks and fermenter of 6 liters, sh owing that cellular dried mass (CDM) increased with the scale up as a consequence of better oxygen transfer. A yield of 1.12 g/l of CDM was achieved utilizing 15 g/l of (NH4)(2)SO4 and 5%(v/v) of ECO in the Erl enmeyer flasks fermentation. A yield of 3.12 g/l was obtained utilizin g 1.0 g/l of NH4Cl and 20%(v/v) of ECO in the six liters fermenter. In this last case the COD was reduced from 6,188 to 4,773mg/l.