TRIMIDOX-MEDIATED MORPHOLOGICAL-CHANGES DURING ERYTHROID-DIFFERENTIATION IS ASSOCIATED WITH THE STIMULATION OF HEMOGLOBIN AND F-CELL PRODUCTION IN HUMAN K562 CELLS
Ew. Iyamu et al., TRIMIDOX-MEDIATED MORPHOLOGICAL-CHANGES DURING ERYTHROID-DIFFERENTIATION IS ASSOCIATED WITH THE STIMULATION OF HEMOGLOBIN AND F-CELL PRODUCTION IN HUMAN K562 CELLS, Biochemical and biophysical research communications (Print), 247(3), 1998, pp. 759-764
Trimidox (3,4,5-trihdroxybenzamidoxime) has been shown to reduce the a
ctivity of ribonucleotide reductase with accompanied growth inhibition
and differentiation of mammalian cells. Hydroxyurea (HU) is the only
ribonucleotide reductase inhibitor in clinical use for the treatment a
nd management of sickle cell anemia, since this compound increases fet
al hemoglobin (Hb F) production: a potent inhibitor of sickle hemoglob
in (Hb SS) polymerization, However, the main limitations of HU is its
lack of potency, myelosuppression and short half life. These studies i
nvestigated the effects of trimidox on the induction of hemoglobin and
F-cells production in K562 erythroleukemia cells. Our study reveals t
hat trimidox exhibits concentration dependent inhibitory effect on K56
2 cells with increase in benzidine positive normoblasts and F-cells pr
oduction as well as morphological changes typical of erythroid differe
ntiation. These findings provide the first evidence that the growth in
hibitory differentiation of cells induced by trimidox enhance hemoglob
in and F-cells production. (C) 1998 Academic Press.