ADENOVIRUS CORE PROTEIN-V INTERACTS WITH P-32 - A PROTEIN WHICH IS ASSOCIATED WITH BOTH THE MITOCHONDRIA AND THE NUCLEUS

Adenovirus protein V is associated with the DNA-containing virus core and functions as a bridge between the capsid and the core. A yeast two -hybrid analysis performed with a human cDNA library using protein V a s 'bait' selected a cellular protein, p32 - described previously as as sociated with the splicing factor ASF/SF2. By expression and purificat ion of p32 and preparation of an antibody we confirmed the binding of p32 to V by a variety of methods including immune precipitation. We de monstrated that p32 was primarily located in the cytoplasm in associat ion with mitochondria but could also be detected in the nucleus as dis tinct granules and tubules. By examining infected cells using confocal microscopy and immunofluorescence we were able to follow the intracel lular locations of protein V and p32 and it is postulated that p32 is part of a system which imports proteins to the nucleus and that adenov irus hijacks this process to deliver its genome to the nucleus.